[Joejoe]

Hi,

Highly appreciated for your answer. I will check them. But, isn’t it low encapsulation efficiency (up to 10%) considering other research articles? Although there are more advanced and expensive materials , even lecithin-mediated encapsulation efficiency can exceed 20%.

Best,

Joe

• This reply was modified 11 months, 4 weeks ago by  Joejoe.

[Joejoe]

I totally agree. Honestly, that is also what we are doing. We have some data on single ingredients performed by suppliers. We use them for claims representing the finished product. But as you said, whatever you find in vitro, it may not be there in vivo. (This was proven many times in pharma. My background is pharmaceuticals actually). I have always been surprised seeing some CROs offering in vitro efficacy testing. I just wanted to know whether there is something that I don’t know. We tried in vitro, but we had problems that I mentioned in my previous comment. I still believe that it is better to run in Vivo with participant trials. Is there any reliable CRO that you can suggest to run participant/ clinical in Vivo efficacy trial with affordable prices 😅?

[Joejoe]

Hi,

Thank you for your reply. I can elaborate my post with an example. Let’s say I have a product and I want to have a claim of “Product X increases collagen production.” based on in vitro assays. Experimentally, there are multiple things can be done such as checking collagen gene expression (qrt pcr), protein level (western blot), immunoflouresence so on and so forth. For such a claim, I need to use the finished product. However, as a nature of the assay, when the finished product is added, it will be diluted when you added into cell culture media etc. Problem 1: there may not be effect there anymore because of dilution. Problem 2: some finished products are not dissolved well (cream etc.) or emulsifier gets broken etc. In that case, how do all those CRO companies offer such tests? How do they do? To my understanding, in vitro tests (especially for claims) should be based on ingredients rather than the finished product. The finished product should be assessed via some participant trials on humans. Can you enlighten me about how the system works in the cosmetic industry? Thanks

Best,

• This reply was modified 1 year, 1 month ago by  Joejoe.