Usefullness and stability vitamin B12 in creamsI would like to try to formulate a cream with vitamin B12 as topical active (eczema).
Vitamin B12 is an effective scavenger of nitric oxide. NO has been found to be implicated in the pathogenesis of (atopic) eczema and psoriasis.
I’ve found a research showing that the experimental application of a NO synthase inhibitor (N omega-nitro-L-arginine), led to a clear decrease in pruritus and erythema in atopic dermatitis, so one would expect a comparable effect from vitamin B12.
Point is which form?
Some researches (the excerpts) don’t mention the form of B12 used.
Some use cyanocobalamine. I also found a simple W/O emulsion formula with it:
· 0.07 g vitamin B12 (cyanocobalamin)· 46 g avocado oil· 45.42 g water· 8 g methyl glucose sesquistearate· 0.26 g calcium sorbate· 0.25 g citric acid
The form I can get hold of is hydroxocobalamine. Cyanocobalamine as well as OH-cobalamine are two biologically inactive forms of B12 and need to be converted via a methylation cycle to be fully functional systemically.
Apparently the conversion doesn’t seem to be necessary to inhibit nitric oxide though, as OH-cobalamine binds and scavenges NO (source).
Since information on topical B12 emulsions (especially with OH-cobalamine) is very scarce, I really hope people here can help me:
·Does anyone know how stable OH-cobalamine is in an emulsion?
·What pH range is necessary for it to remain stable?
·Is a buffer recommended?
·Important interactions (e.g. preservatives)?
·W/O rather than O/W?
I know what I’m planning to formulate would be OTC rather than cosmetic, since I hope it will relieve eczemic laesions. I’m not planning to sell, I will be making it for a family member.
Hopefully it can be succesfully used next to indifferent creams and topical corticosteroids.
Edit: adjusted lay-out
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