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Peptides protocols
Anyone have advice with working with Peptides and how to keep them stable while in storage?
We are getting powdered peptide and wondering if we should reconstitute them in glycerol or just add the peptide straight into the mixture.
Also, when we create the serum emulsions, will homogenization break up the peptides and if so at what stage should we add them?
Thank you!!
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15 Replies
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What do you mean by “stable”? That is, what test would you run or characteristic would you recognize that lets you know whether the peptide is stable?
If the peptide is simply added as a claims ingredient, it doesn’t really matter.
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Keep them refrigerated and add during the cool-down phase at <= 45C.
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@belassi thanks, so don’t reconstitute in glycerol? May be using a homogenizer mixer, would that breakdown the peptide?
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I use liquid peptides, I imagine that it all depends on if the peptides are soluble / dispersible in water.
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Most if not all peptides are soluble/dispersible in water and hydrophilic solvents such as glycerine. Can be added at the end during cool down ie<40C.
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sounds good. Do you a homogenizer to make your emulsions?
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NO we use a high speed overhead mixer and have made many products using peptides some of which are still on the market.Long term stability has never been an issue.
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Interesting, so you use a propeller impeller or coil at 5rpms? I ask cause we are deciding how best to mix the water / oil mix to create our emulsion
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*5000rpm
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1000-2000 RPM is plenty for normal oil/water emulsion.Heat both phases to 70C in seperate beakers and add oil to water for o/w emulsion and mix until homogeneous 10-15 minutes.
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@”DRBOB@VERDIENT.BIZ”
The prices of peptides are volatile, at the moment. Yes, I do agree that a few years ago peptides would have been very expensive to make, but as technology progressed materials or protocols in making peptides had decreased but prices haven’t stabilized or don’t reflect those technology changes.
I could tell cause peptide XYZ is 90 in one company, 250 in another and 175 in another.
What company would you recommend to get solid state (powder) peptides? I prefer not to get liquid form cause the supplier deems the concentration of the peptide in its glycerol mixture a secret, so you can’t really gauge how much actual peptide you have
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If you use Pal-GHK, Syn-ake, Nonapeptide-1, eyeliss peptide or others, we can supply.
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there is a lot of legends on peptide stability that may be explain by the high price, but at the end, in general they are quite stable molecules.
Better to add them at the end of the preparation as most of them are very surface actives. -
I’m still trying to understand what you mean by “stable.” And anyone who has used peptides in formulating can chime in.
What test would you run or characteristic would you recognize that lets you know whether the peptide is stable?
Short of doing x-ray crystallography, how would you tell whether your peptide has kept its conformation or has simply become a hydrolyzed protein?
Also, how can you tell whether the supplier has provided you an actual peptide or just a solution of amino acids in the right proportion of the peptide?
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You have to get a powder standard from the supplier and develop HPLC method for your formula.run and test same at 50C in formula four to six weeks and/or three months at 38C. Argireline (the botox peptide) solution contains 0.05% of the powder which can be obtained from Centerchem wwwcentrdhem.com.https://onlinelibrary.wiley.com/doi/full/10.1111/j.1468-2494.2009.00490.x
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