Home Cosmetic Science Talk Formulating General Antioxidant testing

  • Antioxidant testing

    Posted by Learntounlearn on December 14, 2020 at 3:22 pm

    I attempted to test my cream for its antioxidant potential. So I tried a basic FRAP test (The ferric reducing antioxidant power). Although it’s an assay  I tried a qualitative test to detect if the cream has any antioxidant activity.
    I always got sea green color not the Prussian blue. So I wanted to know if sea green color indicates any antioxidant activity.

    Sorry if all this sounds too amateurish

    Learntounlearn replied 3 years, 6 months ago 2 Members · 2 Replies
  • 2 Replies
  • Pharma

    Member
    December 14, 2020 at 8:32 pm
    It would be better to test pure ingredients rather than a cream. Lipids and emulsifiers can interfere with the assay.
    At least use positive and negative controls = a cream without added antioxidants/herbal extracts and one where you add a fair amount of a known working antioxidant. This would also give you the answer to your Q.
  • Learntounlearn

    Member
    December 15, 2020 at 3:30 am

    Thank you so much. I never thought of this.

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