Color change in closed transparent plastic vials as to closed non transparent serum bottle

Tagged: color-change, formulating, serums

Formulating

Color change in closed transparent plastic vials as to closed non transparent serum bottle

Posted by Iwana on February 23, 2022 at 5:24 pm

Hi Guys,

I was wondering what were your thoughts.

I formulated a serum and put it in the closed non transparent serum bottle the one it is supposed to go in the market in on stability it passed which no color change and no separation at all.

The same formulation I wanted to see outside at room temperature. One of them I put in the same bottle as on stability and the other in a closed transparent plastic vial .
After about two months outside at room temperature I checked the formulation and the one in the closed non transparent serum bottle the one it is supposed to go in the market in it was still good. But on the other hand the one in closed transparent plastic vial had turned dark pink and it had like layer of pink all over at the top. I have attached a picture for you to see.

It serum in the pipette is the one from inside the closed non transparent serum bottle the one it is supposed to go in the market in after the 2 months.

I felt it is light or maybe oxidation. What do you think could it be.

Thank you so much for your help.

evchem2 replied 2 years, 9 months ago 6 Members · 9 Replies
9 Replies

CedarS

Member
February 24, 2022 at 5:20 pm

It could be oxidization and it could be Serratia marcescens (bacteria).
evchem2

Member
February 25, 2022 at 3:21 pm

Without knowing what’s in the formula it’s hard to help, but CedarS makes a good point-have you performed PET on your product?
Graillotion

Member
February 27, 2022 at 6:18 pm

What is the preservative used?
Paprik

Member
February 28, 2022 at 12:23 am

Yeah, first coming on my mind is microbial contamination.
Also acid environment can turn emulsion pink sometimes.

And also, sodium hydroxymethylglycinate can turn formulas pink in the presence of iron.
Abdullah

Member
February 28, 2022 at 1:02 am

Paprik said:

Yeah, first coming on my mind is microbial contamination.
Also acid environment can turn emulsion pink sometimes.
do you know the reason for this color change? One of my samples changed pinkish too and i couldn’t know what was the reason. It was a cationic emulsion.
Paprik

Member
February 28, 2022 at 2:07 am

@Abdullah, hmmm, as it was cationic system, I’m afraid I won’t be able to help. Usually chelating agent helps with this issue.
Did you have enough antioxidant in the formula?
Abdullah

Member
February 28, 2022 at 3:06 am

Paprik said:

@Abdullah, hmmm, as it was cationic system, I’m afraid I won’t be able to help. Usually chelating agent helps with this issue.
Did you have enough antioxidant in the formula?

I didn’t have any antioxidant or chelating agent in that.
The oils were 0.1% rose fragrance oil, 1% olive oil and 4% petrolatum.
Iwana

Member
February 28, 2022 at 7:06 pm

Hi guys thank you for all your responses. I do have preservative in the formulation.

PHENOXYETHANOL, ETHYLHEXYLGLYCERIN (EUXYL 9010) 1.00

Also I do have Chelating agent

Disodium EDTA 0.20

I was think maybe Light.
This formulation contains CBD 0.9.
But this much color change I was wondering. Specially cause of the lines of color.
It got me confused.
evchem2

Member
March 3, 2022 at 2:09 pm

Ah yes if you have CBD isolate in there you will see a pinkish color after light exposure- not sure exactly what it’s degrading to but I saw the same thing when formulating a CBD line. You may not have your CBD as uniformly dispersed as you hope, especially since your oils don’t seem like the best to dissolve the CBD- try MCT oil.