[Microinjala]

Hello everyone! I am new in microbiology and have a lot of question to ask, especially in PET test.

1. How to do a micro challenge test for the W/O cosmetics? I have read the summary of CTFA-6(through this link http://cosmetictestlabs.com/ctfa_m-6_summary.html), but it just mentioned one method as that:”reduced the inoculum volume or oil based carrier system”, I want to know how to reduced the inoculum(centrifuge?)? And oil based carrier means I need to put the microorgnisim into oil? What kind of oil? 

2. CTFA-6 also mentioned molds surface inoculation for W/O soild products, I want to konw how to inoculate and how do the judge? I think spray or inoculate with a disc of media may be practical method, but I do not know if it is right?

3. Do you have good research artical or report to reconmend?

Thank you so much!