Microbial Challenge Test Protocols For Solid Cosmetic Formulations

MarkBroussardMarkBroussard Member, Professional Chemist
I have searched trying to find microbial challenge protocols for products that are solids.  Anyone know what testing techniques are used?

I am assuming it is probably a disc test on a petri plate, where a disc is created from the solid sample, but can't find any definitive information on this.
Chemist/Microbiologist formulating in the Organic & Naturals arena under ECOCert/Natural Products Assn/Whole Foods/National Organic Program guidelines focused skincare & haircare products. 

See website for details www.desertinbloomcosmeticslab.com

Comments

  • BobzchemistBobzchemist Member, PCF student
    In my limited knowledge of how these tests are actually conducted, I am pretty sure that a blender was involved...but that's as far as I can go.
    Robert Zonis, Sr. Formulation Chemist, Beaumont Products "All opinions and comments expressed are my own, have no relation to Beaumont Products, are fully copyrighted, and may not be used without written permission."
  • MarkBroussardMarkBroussard Member, Professional Chemist
    Thanks BobZ.  Perhaps the product is blended into a paste and and the paste is placed into the liquid agar growth medium and the plate is streaked with bacteria.  If there is no growth on the plate, the preservative is working.  Or perhaps the product is blended with the liquid agar and then plated.

    I am only familiar with the disc technique, but that is dated knowledge.
    Chemist/Microbiologist formulating in the Organic & Naturals arena under ECOCert/Natural Products Assn/Whole Foods/National Organic Program guidelines focused skincare & haircare products. 

    See website for details www.desertinbloomcosmeticslab.com
  • WANYINWANYIN Member
    MarkBroussard Hi Mark, I am looking for someone to do some formulations for acne-prone care, do you offer a service for doing formulations? Could you send me an email: xie_shally@yahoo.com, let's see what we can do. Thanks a lot.
  • Chemist77Chemist77 Member, PCF student
    @MarkBroussard I was recently in Dow Microbial Lab and witnessed something about paints and their preservation over a period of years for microbial and algae growth. They used a  patch of paint in the middle of the dish and sprayed with microbes to be tested and separately with algae to check the efficacy of the preservative. 
    I don't know how relevant it is for you but I guess this is how you would go about solids as well and as you mentioned by using a paste of it. But next time I am there I am gonna brig up this point just to make sure. 
  • MarkBroussardMarkBroussard Member, Professional Chemist
    @Chemist77:

    Thanks!  Very much appreciate the comment.  That is about the only technique that I can think of for solids.
    Chemist/Microbiologist formulating in the Organic & Naturals arena under ECOCert/Natural Products Assn/Whole Foods/National Organic Program guidelines focused skincare & haircare products. 

    See website for details www.desertinbloomcosmeticslab.com
  • Chemist77Chemist77 Member, PCF student
    Just an addition, they were doing four cycle challenge test and depending on the customer request they are willing to extend this period further. 

  • We run CTFA M-6 for atypical cosmetics.  There is some info on the linked page.
    For testing help visit us at Cosmetic Test Labs
Sign In or Register to comment.